Journal: Neurotherapeutics

Article Title: Down-regulation of lipocalin-2 alleviates depressive-like behaviors in mice through modulation of microglial activation

doi: 10.1016/j.neurot.2026.e00862

Figure Lengend Snippet: LCN2 neutralizing antibodies alleviated the depressive-like behaviors in CUS mice. ( A ) Schematic of the experimental procedure and behavioral studies. ( B ) Levels of LCN2 measured by ELISA in the mouse hippocampus treated with IgG or anti-LCN2 (n = 6/group). ∗∗∗ P < 0.001 versus the IgG group using Student's t -test. ( C – H ) LCN2 neutralizing antibodies relieved depressive-like behaviors in CUS mice (n = 12/group) as measured by the SPT ( C ), FST ( D) , TST ( E ) and OFT ( F – H ). ∗∗∗ P < 0.001 versus the Control + IgG group; ### P < 0.001 versus the CUS + anti-IgG group using two-way ANOVA followed by the Holm-Sidak post hoc multiple comparison test.

Article Snippet: After incubation in blocking buffer, membranes were incubated overnight at 4 °C with the following primary antibodies: anti-LCN2 (1:1000, abs134038, Absin), anti-iNOS (1:1000, 18985-1-AP, Proteintech Group), anti-p(Ser616)DRP1 (1:1000, ab314755, Abcam), anti-DRP1 (1:1000, 12957-1-AP, Proteintech Group), anti-MFN2(1:1000, 12186-1-AP, Proteintech Group), and anti-GAPDH (1:3000, 60004-1-AP, Proteintech Group).

Techniques: Enzyme-linked Immunosorbent Assay, Control, Comparison

Journal: Neuromolecular medicine

Article Title: Piperlongumine Improves Lipopolysaccharide-Induced Amyloidogenesis by Suppressing NF-KappaB Pathway.

doi: 10.1007/s12017-018-8495-9

Figure Lengend Snippet: Fig. 6 Inhibitory effect of PL on LPS-induced amyloidogen- esis and neuroinflammation in cultured brain cells. The expres- sions of amyloidogenesis pro- teins, such as APP and BACE1, were detected by Western blot- ting using specific antibodies in the astrocyte primary (a) and microglial BV-2 cells (b). The levels of Aβ1−42 in the astrocyte primary (c) and microglial BV-2 cells (d) were measured by ELISA. Data are expressed as the mean ± S.E.M. (t test, *p < .05 vs. control, #p < .05 vs. LPS). The expressions of neuro- inflammation proteins, such as iNOS, COX-2 and GFAP, were detected by Western blotting using specific antibodies in the astrocyte primary (e) in the microglial BV-2 cells (f). Each blot is representative of three experiments

Article Snippet: The sections were reacted with specific primary antibodies for Aβ (1:300; Abcam, Inc., Cambridge, MA, USA), glial fibrillary acidic protein (GFAP) (1:300; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), ionize calcium-binding adapter molecule 1 (IBA-1) (1:300; Abcam, Inc., Cambridge, MA, USA), inducible nitric oxide synthase (iNOS) (1:300; Novus Biologicals, Inc., Littleton) and cyclooxygenase-2 (COX-2) (1:300; Cell Signaling Technology, Inc., Beverly, MA, USA).

Techniques: Cell Culture, Western Blot, Enzyme-linked Immunosorbent Assay, Control

Journal: Italian Journal of Animal Science

Article Title: Protective effect of chitosan oligosaccharide against oxidative damage of peripheral blood mononuclear cells in dairy cows induced by diethylenetriamine/nitric oxide via NF-κB signalling pathway

doi: 10.1080/1828051x.2020.1772131

Figure Lengend Snippet: Figure 2. Effects of chitosan oligosaccharideon on the phosphorylation level of NF-kB pathway, the protein expression level of nitric oxide synthase (iNOS) and interleukin-1b (IL-1b). Expressions of IL-1b(A); phosphorylated IjB kinase b (P-IKKb) (B); phosphorylated inhibitor of nuclear factor kappa-Ba (P-IjBa) (C); phosphorylated nuclear factor kappa-Bp65(P-NF-jBp65) (D); iNOS (E), protein levels were detected by western blotting and normalised to beta-actin (b-Actin) levels. CTR¼ control treatment, without chitosan oligosacchar- ides addition; COS40, COS80, COS160, and COS320¼ treated with 40, 80, 160, and 320lg/mL chitosan oligosaccharides respectively.

Article Snippet: The membranes were incubated in the following primary antibodies: primary polyclonal rabbit anti-iNOS antibody (NBP1-97471, Novus Biological, USA), rabbit anti-IL-1b (Cell Signalling Technology, Boston, MA), anti-IKKb, monoclonal rat anti-IjBa, monoclonal rabbit anti-NF-jBp65 antibody(NF-jB pathway Sampler Kit 9963,CST, MA), anti-phospho-IKKb, anti-phospho-IjBa, and anti-phospho-NF-jBp65 (NF-jB pathway Sampler Kit 9963, CST, MA).

Techniques: Phospho-proteomics, Expressing, Western Blot, Control